MERS-Coronavirus Spike antibody (RBD)

Catalog No. ABIN7596097

The Mouse Monoclonal anti-MERS-Coronavirus Spike antibody has been validated for ELISA and WB. It is suitable to detect MERS-Coronavirus Spike in samples from Middle East Respiratory Syndrome Coronavirus (MERS-CoV).

Quick Overview for MERS-Coronavirus Spike antibody (RBD) (ABIN7596097)

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Reactivity: Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This MERS-Coronavirus Spike antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: AT2F7

Product Details anti-MERS-Coronavirus Spike Antibody

Binding Specificity: RBD

Purpose: MERS-CoV Spike RBD antibody

Purification: protein-A affinity chromatography

Immunogen: Recombinant MERS-CoV Spike RBD (358-606aa) purified from Baculovirus

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for MERS-Coronavirus Spike

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Alternative Name: MERS-CoV Spike

Background: MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.