FASL antibody (AA 196-281)

Catalog No. ABIN726410

The Rabbit Polyclonal anti-FASL antibody has been validated for WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc) and IHC (fro). It is suitable to detect FASL in samples from Human, Mouse, Rat and Pig. There are 9+ publications available.

Quick Overview for FASL antibody (AA 196-281) (ABIN726410)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FASL Antibody

Binding Specificity: AA 196-281

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fas Ligand

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FASL antibody (ABIN726410)

Lv, Zheng, Zhou, Jia, Wang, Liu, Zhao, Ji, Li, Cao: "Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma." in: Scientific reports, Vol. 7, pp. 40967, (2017) (PubMed).

Wang, Zhang, Yang, Luo, Zhang, Wang: "Lipopolysaccharide-induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro-inflammatory cytokines and miR-187." in: Molecular reproduction and development, (2015) (PubMed).

Song, Li, Shi, Lehmler, Fu, Su, Xia, Song, Song: "Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells." in: Chemical research in toxicology, Vol. 28, Issue 11, pp. 2160-9, (2015) (PubMed).

Peng, Fu, Guo, Zhang, Di, Jiang, Li: "Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro." in: Iranian journal of pharmaceutical research : IJPR, Vol. 14, Issue 1, pp. 251-61, (2015) (PubMed).

Qi, Fu, Wang, Liu, Quan, Kourouma, Yan, Yu, Duan, Yang: "BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 50, pp. 108-16, (2014) (PubMed).

Chen, Xie, Xue, Ji, Chang, Ma, Bi: "Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius." in: Research in veterinary science, Vol. 94, Issue 3, pp. 496-503, (2013) (PubMed).

Shi, Song, Wang, Wang, Liang, Hu, Yu, Guan, Cheng, Yang: "β-Benzene hexachloride induces apoptosis of rat Sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL." in: Environmental toxicology, Vol. 26, Issue 2, pp. 124-35, (2011) (PubMed).

Shi, Song, Wang, Liang, Hu, Guan, Cheng, Yang: "p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway." in: Journal of biomedicine & biotechnology, Vol. 2009, pp. 181282, (2009) (PubMed).

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: Fas Ligand

Background:

Synonyms: APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, Fas ligand, FASLG

Background: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity