ADAR antibody (AA 1-1152)

Catalog No. ABIN7598956

The Rabbit Polyclonal anti-ADAR antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect ADAR in samples from Human, Mouse and Rat.

Quick Overview for ADAR antibody (AA 1-1152) (ABIN7598956)

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ADAR Antibody

Binding Specificity: AA 1-1152

Purpose: Anti-ADAR1/ADAR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADAR1/ADAR Antibody Picoband® (ABIN7598956). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ADAR1/ADAR recombinant protein (Position: M1-E1152).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Agranat, L., Raitskin, O., Sperling, J., Sperling, R. The editing enzyme ADAR1 and the mRNA surveillance protein hUpf1 interact in the cell nucleus. Proc. Nat. Acad. Sci. 105: 5028-5033, 2008. 2. Bass, B. L., Weintraub, H. An unwinding activity that covalently modifies its double-stranded RNA substrate. Cell 55: 1089-1098, 1988. 3. Chao, S.-C., Huang, C.-Y., Yang, M.-H. A novel nonsense mutation of the DSRAD gene in a Taiwanese family with dyschromatosis symmetrica hereditaria. Europ. J. Derm. 16: 449-540, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ADAR

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: ADAR

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137, Tnfrsf9, Cd137, Ila, Ly63

Tissue Specificity: Expressed on the surface of activated T-cells.

Background: Double-stranded RNA-specific adenosine deaminase is an enzyme that in humans is encoded by the ADAR gene. This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Molecular Weight: 110 kDa

Gene ID: 103

UniProt: P55265

Pathways: Protein targeting to Nucleus