TKT antibody (AA 1-116)

Catalog No. ABIN7598957

This anti-TKT antibody is a Mouse Monoclonal antibody detecting TKT in WB, IHC, IF, FACS and ICC. Suitable for Human, Rat and Mouse.

Quick Overview for TKT antibody (AA 1-116) (ABIN7598957)

Target: TKT (Transketolase (TKT))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TKT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2I3

Product Details anti-TKT Antibody

Binding Specificity: AA 1-116

Purpose: Anti-Transketolase/TKT Antibody (monoclonal, 2I3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Transketolase/TKT Antibody (monoclonal, 2I3) (ABIN7598957). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Transketolase/TKT recombinant protein (Position: M1-A116).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TKT

Target: TKT (Transketolase (TKT))

Alternative Name: TKT

Background:

Synonyms: Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is ly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.

Molecular Weight: 68 kDa

Gene ID: 7086

UniProt: P29401