Id2 antibody (AA 1-133)

Catalog No. ABIN7598980

The Rabbit Polyclonal anti-Id2 antibody has been validated for ELISA and WB. It is suitable to detect Id2 in samples from Human, Rat and Mouse.

Quick Overview for Id2 antibody (AA 1-133) (ABIN7598980)

Target: Id2 (Inhibitor of DNA Binding 2 (Id2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Id2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-Id2 Antibody

Binding Specificity: AA 1-133

Purpose: Anti-ID2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ID2 Antibody Picoband® (ABIN7598980). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ID2 recombinant protein (Position: M1-C133).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Langlands K, Yin X, Anand G, Prochownik EV (Aug 1997). "Differential interactions of Id proteins with basic-helix-loop-helix transcription factors". J. Biol. Chem. 272 (32): 19785-93. 2. Law SF, Zhang YZ, Fashena SJ, Toby G, Estojak J, Golemis EA (Oct 1999). "Dimerization of the docking/adaptor protein HEF1 via a carboxy-terminal helix-loop-helix domain". Exp. Cell Res. 252 (1): 224-35.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Id2

Target: Id2 (Inhibitor of DNA Binding 2 (Id2))

Alternative Name: ID2

Background:

Synonyms: DNA-binding protein inhibitor ID-2,Class B basic helix-loop-helix protein 26,bHLHb26,Inhibitor of DNA binding 2,Inhibitor of differentiation 2,ID2,BHLHB26,

Tissue Specificity: Highly expressed in early fetal tissues, including those of the central nervous system.

Background: DNA-binding protein inhibitor ID-2 is a protein that in humans is encoded by the ID2 gene. The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.

Molecular Weight: 18 kDa

Gene ID: 3398

UniProt: Q02363