CBX5 antibody (AA 1-191)

Catalog No. ABIN7599057

This anti-CBX5 antibody is a Mouse Monoclonal antibody detecting CBX5 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for CBX5 antibody (AA 1-191) (ABIN7599057)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 8G6

Product Details anti-CBX5 Antibody

Binding Specificity: AA 1-191

Purpose: Anti-HP1 alpha/CBX5 Antibody Picoband® (monoclonal, 8G6)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HP1 alpha/CBX5 Antibody Picoband® (monoclonal, 8G6) (ABIN7599057). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HP1 alpha/CBX5 recombinant protein (Position: M1-S191).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Allan, R. S., Zueva, E., Cammas, F., Schreiber, H. A., Masson, V., Belz, G. T., Roche, D., Maison, C., Quivy, J.-P., Almouzni, G., Amigorena, S. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature 487: 249-253, 2012. 2. Bannister, A. J., Zegerman, P., Partridge, J. F., Miska, E. A., Thomas, J. O., Allshire, R. C., Kouzarides, T. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410: 120-124, 2001. 3. Cheutin, T., McNairn, A. J., Jenuwein, T., Gilbert, D. M., Singh, P. B., Misteli, T. Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science 299: 721-725, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: CBX5

Background:

Synonyms: N-myc-interactor, Nmi, N-myc and STAT interactor, NMI

Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Background: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 22 kDa

Gene ID: 23468

UniProt: P45973