IFT43 antibody (AA 1-208)

Catalog No. ABIN7599083

The Rabbit Polyclonal anti-IFT43 antibody has been validated for WB, ELISA, FACS, ICC and IF. It is suitable to detect IFT43 in samples from Human, Mouse and Rat.

Quick Overview for IFT43 antibody (AA 1-208) (ABIN7599083)

Target: IFT43 (Intraflagellar Transport 43 Homolog (IFT43))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT43 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IFT43 Antibody

Binding Specificity: AA 1-208

Purpose: Anti-IFT43 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IFT43 Antibody Picoband® (ABIN7599083). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IFT43 recombinant protein (Position: M1-T208).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arts, H. H., Bongers, E. M. H. F., Mans, D. A., van Beersum, S. E. C., Oud, M. M., Bolat, E., Spruijt, L., Cornelissen, E. A. M., Schuurs-Hoeijmakers, J. H. M., de Leeuw, N., Cormier-Daire, V., Brunner, H. G., Knoers, N. V. A. M., Roepman, R. C14ORF179 encoding IFT43 is mutated in Sensenbrenner syndrome. J. Med. Genet. 48: 390-395, 2011. 2. Biswas, P., Duncan, J. L., Ali, M., Matsui, H., Naeem, M. A., Raghavendra, P. B., Frazer, K. A., Arts, H. H., Riazuddin, S., Akram, J., Hejtmancik, J. F., Riazuddin, S. A., Ayyagari, R. A mutation in IFT43 causes non-syndromic recessive retinal degeneration. Hum. Molec. Genet. 26: 4741-4751, 2017. 3. Duran, I., Taylor, S. P., Zhang, W., Martin, J., Qureshi, F., Jacques, S. M., Wallerstein, R., Lachman, R. S., Nickerson, D. A., Bamshad, M., Cohn, D. H., Krakow, D. Mutations in IFT-A satellite core component genes IFT43 and IFT121 produce short rib polydactyly syndrome with distinctive campomelia. Cilia 6: 7, 2017. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IFT43

Target: IFT43 (Intraflagellar Transport 43 Homolog (IFT43))

Alternative Name: IFT43

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 23 kDa

Gene ID: 112752

Pathways: Hedgehog Signaling