Tppp antibody (AA 1-219)

Catalog No. ABIN7599102

This anti-Tppp antibody is a Rabbit Polyclonal antibody detecting Tppp in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Tppp antibody (AA 1-219) (ABIN7599102)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tppp antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Tppp Antibody

Binding Specificity: AA 1-219

Purpose: Anti-TPPP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TPPP Antibody Picoband® (ABIN7599102). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TPPP recombinant protein (Position: M1-K219).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Martin, C. P., Vazquez, J., Avila, J., Moreno, F. J. P24, a glycogen synthase kinase 3 (GSK 3) inhibitor. Biochim. Biophys. Acta 1586: 113-122, 2002. 2. Seki, N., Hattori, A., Sugano, S., Suzuki, Y., Nakagawara, A., Muramatsu, M., Hori, T., Saito, T. A novel human gene whose product shares significant homology with the bovine brain-specific protein p25 on chromosome 5p15.3. J. Hum. Genet. 44: 121-122, 1999. 3. Tirian, L., Hlavanda, E., Olah, J., Horvath, I., Orosz, F., Szabo, B., Kovacs, J., Szabad, J., Ovadi, J. TPPP/p25 promotes tubulin assemblies and blocks mitotic spindle formation. Proc. Nat. Acad. Sci. 100: 13976-13981, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Alternative Name: TPPP

Background:

Synonyms: "Long-chain-fatty-acid--CoA ligase 4, Long-chain acyl-CoA synthetase 4, LACS 4, ACSL4, ACS4, FACL4, LACS4 "

Tissue Specificity: Expressed in the prostate gland and prostate cancers.

Background: Tubulin polymerization-promoting protein is a protein that in humans is encoded by the TPPP gene. Enables several functions, including GTPase activity, magnesium ion binding activity, and protein homodimerization activity. Involved in several processes, including microtubule cytoskeleton organization, negative regulation of tubulin deacetylation, and positive regulation of protein polymerization. Located in several cellular components, including mitochondrion, mitotic spindle, and perinuclear region of cytoplasm. Colocalizes with microtubule and microtubule bundle.

Molecular Weight: 24 kDa

Gene ID: 11076

UniProt: O94811