NDRG4 antibody (AA 1-226)

Catalog No. ABIN7599112

This Rabbit Polyclonal antibody specifically detects NDRG4 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NDRG4 antibody (AA 1-226) (ABIN7599112)

Target: NDRG4 (NDRG Family Member 4 (NDRG4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDRG4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NDRG4 Antibody

Binding Specificity: AA 1-226

Purpose: Anti-NDRG4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NDRG4 Antibody Picoband® (ABIN7599112). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NDRG4 recombinant protein (Position: M1-R226).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hirosawa, M., Nagase, T., Ishikawa, K., Kikuno, R., Nomura, N., Ohara, O. Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. DNA Res. 6: 329-336, 1999. 2. Hongo, S., Watanabe, T., Takahashi, K., Miyazaki, A. Ndrg4 enhances NGF-induced ERK activation uncoupled with Elk-1 activation. J. Cell. Biochem. 98: 185-193, 2006. 3. Linhares, N. D., Freire, M. C. M., Cardenas, R. G. C. C. L., Pena, H. B., Bahia, M., Pena, S. D. J. Exome sequencing identifies a novel homozygous variant in NDRG4 in a family with infantile myofibromatosis. Europ. J. Med. Genet. 57: 643-648, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NDRG4

Target: NDRG4 (NDRG Family Member 4 (NDRG4))

Alternative Name: NDRG4

Background:

Synonyms: Transmembrane protein 107,TMEM107,DC20, UNQ638/PRO1268,

Tissue Specificity: Ubiquitous. .

Background: Protein NDRG4 is a protein that in humans is encoded by the NDRG4 gene. This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.

Molecular Weight: 37 kDa

Gene ID: 65009