MED7 antibody (AA 1-233)

Catalog No. ABIN7599129

The Rabbit Polyclonal anti-MED7 antibody (ABIN7599129) specifically detects MED7 in WB, ELISA, ICC, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MED7 antibody (AA 1-233) (ABIN7599129)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-MED7 Antibody

Binding Specificity: AA 1-233

Purpose: Anti-MED7 Antibody

Characteristics: Anti-MED7 Antibody (ABIN7599129). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED7 recombinant protein (Position: M1-P233). Human MED7 shares 97% amino acid (aa) sequence identity with mouse MED7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7

Background: Mediator of RNA polymerase II transcription subunit 7 is an enzyme that in humans is encoded by the MED7 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 27 kDa

Gene ID: 9443

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha