ALAS1 antibody (AA 1-244)

Catalog No. ABIN7599146

The Rabbit Polyclonal anti-ALAS1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ALAS1 in samples from Human, Mouse and Rat.

Quick Overview for ALAS1 antibody (AA 1-244) (ABIN7599146)

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ALAS1 Antibody

Binding Specificity: AA 1-244

Purpose: Anti-Alas1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Alas1 Antibody Picoband® (ABIN7599146). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Alas1 recombinant protein (Position: M1-Q244).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Astrin, K. H., Bishop, D. F. Assignment of human erythroid delta-aminolevulinate synthase (ALAS2) to the X chromosome. (Abstract) Cytogenet. Cell Genet. 51: 953-954, 1989. 2. Bawden, M. J., Borthwick, I. A., Healy, H. M., Morris, C. P., May, B. K., Elliott, W. H. Sequence of human 5-aminolevulinate synthase cDNA. Nucleic Acids Res. 15: 8563 only, 1987. 3. Bishop, D. F., Henderson, A. S., Astrin, K. H. Human delta-aminolevulinate synthase: assignment of the housekeeping gene to 3p21 and the erythroid-specific gene to the X chromosome. Genomics 7: 207-214, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALAS1

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: ALAS1

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme, a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 71 kDa

Gene ID: 211

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha