YWHAE antibody (AA 1-255)

Catalog No. ABIN7599165

This anti-YWHAE antibody is a Mouse Monoclonal antibody detecting YWHAE in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for YWHAE antibody (AA 1-255) (ABIN7599165)

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This YWHAE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3G11G2

Product Details anti-YWHAE Antibody

Binding Specificity: AA 1-255

Purpose: Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) (ABIN7599165). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human YWHAE recombinant protein (Position: M1-Q255).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat
1. Bi, W., Sapir, T., Shchelochkov, O. A., Zhang, F., Withers, M. A., Hunter, J. V., Levy, T., Shinder, V., Peiffer, D. A., Gunderson, K. L., Nezarati, M. M., Shotts, V. A., and 13 others. Increased LIS1 expression affects human and mouse brain development. Nature Genet. 41: 168-177, 2009. 2. Cardoso, C., Leventer, R. J., Ward, H. L., Toyo-oka, K., Chung, J., Gross, A., Martin, C. L., Allanson, J., Pilz, D. T., Olney, A. H., Mutchinick, O. M., Hirotsune, S., Wynshaw-Boris, A., Dobyns, W. B., Ledbetter, D. H. Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Am. J. Hum. Genet. 72: 918-930, 2003. 3. Chong, S. S., Tanigami, A., Roschke, A. V., Ledbetter, D. H. 14-3-3-epsilon has no homology to LIS1 and lies telomeric to it on chromosome 17p13.3 outside the Miller-Dieker syndrome chromosome region. Genome Res. 6: 735-741, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: YWHAE

Background:

Synonyms: A32 antigen antibody, CD 146 antibody, CD146 antibody, CD146 antigen antibody, Cell surface glycoprotein MUC18 antibody, Cell surface glycoprotein P1H12 antibody, Gicerin antibody, MCAM antibody, Melanoma adhesion molecule antibody, Melanoma associated antigen A32 antibody, Melanoma associated antigen MUC18 antibody, Melanoma associated glycoprotein MUC18 antibody, Melanoma cell adhesion molecule antibody, Melanoma-associated antigen A32 antibody, Melanoma-associated antigen MUC18 antibody, MelCAM antibody, MUC 18 antibody, MUC18 antibody, MUC18_HUMAN antibody, S endo 1 antibody, S endo 1 endothelial associated antigen antibody, S-endo 1 endothelial-associated antigen antibody, Sendo 1 endothelial associated antigen antibody, Sendo1 antibody

Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Background: 14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Molecular Weight: 29 kDa

Gene ID: 7531

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase