OVOL2 antibody (AA 1-271)

Catalog No. ABIN7599195

This anti-OVOL2 antibody is a Rabbit Polyclonal antibody detecting OVOL2 in WB, ELISA, IF, ICC and FACS. Suitable for Human.

Quick Overview for OVOL2 antibody (AA 1-271) (ABIN7599195)

Target: OVOL2 (Ovo-Like 2 (OVOL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OVOL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-OVOL2 Antibody

Binding Specificity: AA 1-271

Purpose: Anti-OVOL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-OVOL2 Antibody Picoband® (ABIN7599195). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OVOL2 recombinant protein (Position: M1-E271).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Davidson, A. E., Liskova, P., Evans, C. J., Dudakova, L., Noskova, L., Pontikos, N., Hartmannova, H., Hodanova, K., Stranecky, V., Kozmik, Z., Levis, H. J., Idigo, N., and 14 others. Autosomal-dominant corneal endothelial dystrophies CHED1 and PPCD1 are allelic disorders caused by non-coding mutations in the promoter of OVOL2. Am. J. Hum. Genet. 98: 75-89, 2016. 2. Gwilliam, R., Liskova, P., Filipec, M., Kmoch, S., Jirsova, K., Huckle, E. J., Stables, C. L., Bhattacharya, S. S., Hardcastle, A. J., Deloukas, P., Ebenezer, N. D. Posterior polymorphous corneal dystrophy in Czech families maps to chromosome 20 and excludes the VSX1 gene. Invest. Ophthal. Vis. Sci. 46: 4480-4484, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 6/26/2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for OVOL2

Target: OVOL2 (Ovo-Like 2 (OVOL2))

Alternative Name: OVOL2

Background:

Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16

Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Background: This gene encodes a member of the evolutionarily conserved ovo-like protein family. Mammalian members of this family contain a single zinc finger domain composed of a tetrad of C2H2 zinc fingers with variable N- and C-terminal extensions that contain intrinsically disordered domains. Members of this family are involved in epithelial development and differentiation. Knockout of this gene in mouse results in early embryonic lethality with phenotypes that include neurectoderm expansion, impaired vascularization, and heart anomalies. In humans, allelic variants of this gene have been associated with posterior polymorphous corneal dystrophy.

Molecular Weight: 37 kDa

Gene ID: 58495

Pathways: Tube Formation