RPLP0 antibody (AA 1-271)

Catalog No. ABIN7599197

The Rabbit Polyclonal anti-RPLP0 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect RPLP0 in samples from Human.

Quick Overview for RPLP0 antibody (AA 1-271) (ABIN7599197)

Target: RPLP0 (Ribosomal Protein, Large, P0 (RPLP0))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPLP0 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-RPLP0 Antibody

Binding Specificity: AA 1-271

Purpose: Anti-RPLP0 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RPLP0 Antibody Picoband® (ABIN7599197). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPLP0 recombinant protein (Position: M1-D271).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/25/2016. 2. Rich, B. E., Steitz, J. A. Human acidic ribosomal phosphoproteins P0, P1, and P2: analysis of cDNA clones, in vitro synthesis, and assembly. Molec. Cell. Biol. 7: 4065-4074, 1987. 3. Zou, S. W., Zhang, J. C., Zhang, X. D., Miao, S. Y., Zong, S. D., Sheng, Q., Wang, L. F. Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis. Cell Res. 13: 171-177, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RPLP0

Target: RPLP0 (Ribosomal Protein, Large, P0 (RPLP0))

Alternative Name: RPLP0

Background:

Synonyms: Vasoactive intestinal polypeptide receptor 1, VIP-R-1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R-2, PACAP-R2, VPAC1, VIPR1

Tissue Specificity: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT.

Background: 60S acidic ribosomal protein P0 is a protein that in humans is encoded by the RPLP0 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist, they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Molecular Weight: 37 kDa

Gene ID: 6175

UniProt: P05388