RASD1 antibody (AA 1-281)

Catalog No. ABIN7599211

This Rabbit Polyclonal antibody specifically detects RASD1 in WB, ELISA and FACS. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for RASD1 antibody (AA 1-281) (ABIN7599211)

Target: RASD1 (RAS, Dexamethasone-Induced 1 (RASD1))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RASD1 Antibody

Binding Specificity: AA 1-281

Purpose: Anti-Dexras1/RASD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Dexras1/RASD1 Antibody Picoband® (ABIN7599211). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Dexras1/RASD1 recombinant protein (Position: M1-S281).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cheng, H.-Y. M., Obrietan, K., Cain, S. W., Lee, B. Y., Agostino, P. V., Joza, N. A., Harrington, M. E., Ralph, M. R., Penninger, J. M. Dexras1 potentiates photic and suppresses nonphotic responses of the circadian clock. Neuron 43: 715-728, 2004. 2. Fang, M., Jaffrey, S. R., Sawa, A., Ye, K., Luo, X., Snyder, S. H. Dexras1: a G protein specifically coupled to neuronal nitric oxide synthase via CAPON. Neuron 28: 183-193, 2000. 3. Gross, M. B. Personal Communication. Baltimore, Md. 10/30/2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RASD1

Target: RASD1 (RAS, Dexamethasone-Induced 1 (RASD1))

Alternative Name: RASD1

Background:

Synonyms: Suppressor of cytokine signaling 5, SOCS-5, Cytokine-inducible SH2 protein 6, CIS-6, Cytokine-inducible SH2-containing protein 5, SOCS5, CIS6, CISH5, CISH6, KIAA0671

Tissue Specificity: Widely expressed. Expressed in TH1 but not TH2 cells.

Background: Dexamethasone-induced Ras-related protein 1 (RASD1) is a protein that in humans is encoded by the RASD1 gene on chromosome 17. This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65, which interacts with the Alzheimer's disease amyloid precursor protein. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 32 kDa

Gene ID: 51655

UniProt: Q9Y272