LYAR antibody (AA 1-379)

Catalog No. ABIN7599330

This anti-LYAR antibody is a Rabbit Polyclonal antibody detecting LYAR in WB, ELISA, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for LYAR antibody (AA 1-379) (ABIN7599330)

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-LYAR Antibody

Binding Specificity: AA 1-379

Purpose: Anti-LYAR Antibody Picoband®

Characteristics: Anti-LYAR Antibody Picoband® (ABIN7599330). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LYAR recombinant protein (Position: M1-K379). Human LYAR shares 71.9% and 73.7% amino acid (aa) sequence identity with mouse and rat LYAR, respectively.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 9/21/2017. 2. Ju, J., Wang, Y., Liu, R., Zhang, Y., Xu, Z., Wang, Y., Wu, Y., Liu, M., Cerruti, L., Zou, F., Ma, C., Fang, M., Tan, R., Jane, S. M., Zhao, Q. Human fetal globin gene expression is regulated by LYAR. Nucleic Acids Res. 42: 9740-9752, 2014. 3. Su, L., Hershberger, R. J., Weissman, I. L. LYAR, a novel nucleolar protein with zinc finger DNA-binding motifs, is involved in cell growth regulation. Genes Dev. 7: 735-748, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LYAR

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Alternative Name: LYAR

Target Type: Antibody

Background: Cell growth-regulating nucleolar protein is a protein that in humans is encoded by the LYAR gene (Ly-1 antibody reactive clone). LYAR (Ly-1/CD5 antibody reactive clone) is a 45-50 kDa nucleolar protein that was named for the ability of its antibody to cross-react with Ly-1. Its function is unclear, it is known to associate with MYCN and RRP1B, the latter association giving rise to the suggestion that LYAR is involved with RNA metabolism. Human LYAR is 379 amino acids (aa) in length. It contains two C2H2-type Zn finger regions (aa 6-25 and 33-51) followed by one coiled-coil region (aa 175-219) and an NLS (aa 217-222). There are multiple Zn-binding sites and three utilized phosphorylation sites at Ser244, Ser258 and Ser276. Over aa 288-379, human LYAR shares 75 % aa identity with mouse LYAR.

Molecular Weight: 44 kDa

Gene ID: 55646