NLE1 antibody (AA 1-385)

Catalog No. ABIN7599337

This Rabbit Polyclonal antibody specifically detects NLE1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NLE1 antibody (AA 1-385) (ABIN7599337)

Target: NLE1 (Notchless Homolog 1 (NLE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NLE1 Antibody

Binding Specificity: AA 1-385

Purpose: Anti-NLE1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NLE1 Antibody Picoband® (ABIN7599337). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NLE1 recombinant protein (Position: M1-R385). Human NLE1 shares 94.3% amino acid (aa) sequence identity with mouse NLE1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Beck-Cormier, S., Escande, M., Souilhol, C., Vandormael-Pournin, S., Sourice, S., Pilet, P., Babinet, C., Cohen-Tannoudji, M. Notchless is required for axial skeleton formation in mice. PLoS One 9: e98507, 2014. 2. Gross, M. B. Personal Communication. Baltimore, Md. 8/20/2024. 3. Stanchi, F., Bertocco, E., Toppo, S., Dioguardi, R., Simionati, B., Cannata, N., Zimbello, R., Lanfranchi, G., Valle, G. Characterization of 16 novel human genes showing high similarity to yeast sequences. Yeast 18: 69-80, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NLE1

Target: NLE1 (Notchless Homolog 1 (NLE1))

Alternative Name: NLE1

Background:

Synonyms: NLE1, HUSSY-07, Notchless protein homolog 1

Background: Notchless protein homolog 1 is a protein that in humans is encoded by the NLE1 gene. Predicted to be involved in Notch signaling pathway and ribosomal large subunit assembly. Predicted to act upstream of or within several processes, including chordate embryonic development, hematopoietic stem cell homeostasis, and regulation of signal transduction. Located in nucleolus and nucleoplasm.

Molecular Weight: 45 kDa

Gene ID: 54475