KLF4 antibody (AA 1-389)

Catalog No. ABIN7599343

This Rabbit Polyclonal antibody specifically detects KLF4 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KLF4 antibody (AA 1-389) (ABIN7599343)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-KLF4 Antibody

Binding Specificity: AA 1-389

Purpose: Anti-KLF4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KLF4 Antibody Picoband® (ABIN7599343). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KLF4 recombinant protein (Position: M1-H389).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Moore, D. L., Blackmore, M. G., Hu, Y., Kaestner, K. H., Bixby, J. L., Lemmon, V. P., Goldberg, J. L. KLF family members regulate intrinsic axon regeneration ability. Science 326: 298-301, 2009. 2. Patel, S., Xi, Z. F., Seo, E. Y., McGaughey, D., Segre, J. A. Klf4 and corticosteroids activate an overlapping set of transcriptional targets to accelerate in utero epidermal barrier acquisition. Proc. Nat. Acad. Sci. 103: 18668-18673, 2006. 3. Wang, H., Yang, L., Jamaluddin, M. S., Boyd, D. D. The Kruppel-like KLF4 transcription factor, a novel regulator of urokinase receptor expression, drives synthesis of this binding site in colonic crypt luminal surface epithelial cells. J. Biol. Chem. 279: 22674-22683, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background:

Synonyms: Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF

Background: Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Molecular Weight: 60 kDa

Gene ID: 9314

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance