NOB1 antibody (AA 1-389)

Catalog No. ABIN7599344

The Rabbit Polyclonal anti-NOB1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect NOB1 in samples from Human, Mouse and Rat.

Quick Overview for NOB1 antibody (AA 1-389) (ABIN7599344)

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NOB1 Antibody

Binding Specificity: AA 1-389

Purpose: Anti-NOB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOB1 Antibody Picoband® (ABIN7599344). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOB1 recombinant protein (Position: M1-D389).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Fatica, A., Oeffinger, M., Dlakic, M., Tollervey, D. Nob1p is required for cleavage of the 3-prime end of 18S rRNA. Molec. Cell. Biol. 23: 1798-1807, 2003. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/6/2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOB1

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Alternative Name: NOB1

Background:

Synonyms: Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2, LMO2, RBTN2, RBTNL1, RHOM2, TTG2

Background: RNA-binding protein NOB1 is a protein that in humans is encoded by the NOB1 gene. In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.

Molecular Weight: 47 kDa

Gene ID: 28987

Pathways: Protein targeting to Nucleus