TRAIP antibody (AA 1-469)

Catalog No. ABIN7599419

Product Details anti-TRAIP Antibody

Target: TRAIP (TRAF Interacting Protein (TRAIP))

Binding Specificity: AA 1-469

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAIP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Anti-TRIP/TRAIP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIP/TRAIP Antibody Picoband® (ABIN7599419). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRIP/TRAIP recombinant protein (Position: M1-S469).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 2/2/2016. 2. Harley, M. E., Murina, O., Leitch, A., Higgs, M. R., Bicknell, L. S., Yigit, G., Blackford, A. N., Zlatanou, A., Mackenzie, K. J., Reddy, K., Halachev, M., McGlasson, S., and 18 others. TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nature Genet. 48: 36-43, 2016. 3. Lee, S. Y., Lee, S. Y., Choi, Y. TRAF-interacting protein (TRIP): a novel component of the tumor necrosis factor receptor (TNFR)- and CD30-TRAF signaling complexes that inhibits TRAF2-mediated NF-kappa-B activation. J. Exp. Med. 185: 1275-1285, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRAIP

Target: TRAIP (TRAF Interacting Protein (TRAIP))

Alternative Name: TRAIP (TRAIP Products)

Background:

Synonyms: DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1

Tissue Specificity: Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Background: TRAF-interacting protein is a protein that in humans is encoded by the TRAIP gene. This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.

Molecular Weight: 53 kDa

Gene ID: 10293