KLC1 antibody (AA 1-537)

Catalog No. ABIN7599469

This Rabbit Polyclonal antibody specifically detects KLC1 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for KLC1 antibody (AA 1-537) (ABIN7599469)

Target: KLC1 (Kinesin Light Chain 1 (KLC1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-KLC1 Antibody

Binding Specificity: AA 1-537

Purpose: Anti-KLC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KLC1 Antibody Picoband® (ABIN7599469). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KLC1 recombinant protein (Position: M1-D537).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bracale, A., Cesca, F., Neubrand, V. E., Newsome, T. P., Way, M., Schiavo, G. Kidins220/ARMS is transported by a kinesin-1-based mechanism likely to be involved in neuronal differentiation. Molec. Biol. Cell 18: 142-152, 2007. 2. Cabeza-Arvelaiz, Y., Shih, L.-C. N., Hardman, N., Asselbergs, F., Bilbe, G., Schmitz, A., White, B., Siciliano, M. J., Lachman, L. B. Cloning and genetic organization of the human kinesin light-chain (KLC) gene. DNA Cell Biol. 12: 881-892, 1993. 3. Chernajovsky, Y., Brown, A., Clark, J. Human kinesin light (beta) chain gene: DNA sequence and functional characterization of its promoter and first exon. DNA Cell Biol. 15: 965-974, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KLC1

Target: KLC1 (Kinesin Light Chain 1 (KLC1))

Alternative Name: KLC1

Background:

Synonyms: Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hGC-1, hOLfD, OLFM4, GW112, UNQ362, PRO698

Tissue Specificity: Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Background: Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene. Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos, however, the full-length nature and/or biological validity of most of these variants have not been determined.

Molecular Weight: 65-75 kDa

Gene ID: 3831

UniProt: Q07866

Pathways: Ribonucleoprotein Complex Subunit Organization