RTKN2 antibody (AA 1-572)

Catalog No. ABIN7599487

This Rabbit Polyclonal antibody specifically detects RTKN2 in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RTKN2 antibody (AA 1-572) (ABIN7599487)

Target: RTKN2 (Rhotekin 2 (RTKN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RTKN2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-RTKN2 Antibody

Binding Specificity: AA 1-572

Purpose: Anti-RTKN2 Antibody Picoband®

Characteristics: Anti-RTKN2 Antibody Picoband® (ABIN7599487). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTKN2 recombinant protein (Position: M1-D572). Human RTKN2 shares 71.8% amino acid (aa) sequence identity with mouse RTKN2.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
. Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Collier, F. M., Gregorio-King, C. C., Gough, T. J., Talbot, C. D., Walder, K., Kirkland, M. A. Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2. Biochem. Biophys. Res. Commun. 324: 1360-1369, 2004. 2. Collier, F. M., Loving, A., Baker, A. J., McLeod, J., Walder, K., Kirkland, M. A. RTKN2 induces NF-kappaB dependent resistance to intrinsic apoptosis in HEK cells and regulates BCL-2 genes in human CD4+ lymphocytes. J. Cell Death 2: 9-23, 2009. Note: Electronic Article. 3. Gregorio-King, C. C., Gough, T., Van Der Meer, G. J., Hosking, J. B., Waugh, C. M., McLeod, J. L., Collier, F. M., Kirkland, M. A. Mechanisms of resistance to the cytotoxic effects of oxysterols in human leukemic cells. J. Steroid Biochem. Molec. Biol. 88: 311-320, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RTKN2

Target: RTKN2 (Rhotekin 2 (RTKN2))

Alternative Name: RTKN2

Background: Rhotekin 2 is a protein in humans that is encoded by the RTKN2 gene. The RTKN2 gene encodes a protein known as Rhotekin-2, which is involved in various cellular processes, including cytoskeletal organization, cell migration, and signal transduction. RTKN2 interacts with Rho GTPases, particularly RhoA, and modulates their activity to regulate actin cytoskeleton dynamics and cell morphology. This protein plays a role in cell adhesion and migration, with implications for tissue development, wound healing, and cancer metastasis. Additionally, RTKN2 has been linked to synaptic plasticity and neuronal development in the brain. Dysregulation of RTKN2 expression or function has been associated with neurological disorders, cardiovascular diseases, and cancer progression. Elucidating the molecular mechanisms underlying RTKN2's diverse functions is crucial for understanding its physiological roles and its potential as a therapeutic target for various diseases.

Molecular Weight: 69 kDa

Gene ID: 219790