MCM9 antibody (AA 1-644)

Catalog No. ABIN7599513

Product Details anti-MCM9 Antibody

Target: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Binding Specificity: AA 1-644

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM9 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-MCM9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCM9 Antibody Picoband® (ABIN7599513). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCM9 recombinant protein (Position: M1-L644). Human MCM9 shares 92.7% and 93% amino acid (aa) sequence identity with mouse and rat MCM9, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, Human
1. Fauchereau, F., Shalev, S., Chervinsky, E., Beck-Fruchter, R., Legois, B., Fellous, M., Caburet, S., Veitia, R. A. A non-sense MCM9 mutation in a familial case of primary ovarian insufficiency. Clin. Genet. 89: 603-607, 2016. 2. Lutzmann, M., Grey, C., Traver, S., Ganier, O., Maya-Mendoza, A., Ranisavljevic, N., Bernex, F., Nishiyama, A., Montel, N., Gavois, E., Forichon, L., de Massy, B., Mechali, M. MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination. Molec. Cell 47: 523-534, 2012. 3. Lutzmann, M., Maiorano, D., Mechali, M. Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family. Gene 362: 51-56, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MCM9

Target: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Alternative Name: MCM9 (MCM9 Products)

Background:

Synonyms: MCM9, C6orf61, MCMDC1, DNA helicase MCM9, hMCM9, EC 3.6.4.12, Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9

Background: The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1.

Molecular Weight: 127 kDa

Gene ID: 254394