MTA2 antibody (AA 1-668)

Catalog No. ABIN7599519

This anti-MTA2 antibody is a Rabbit Polyclonal antibody detecting MTA2 in WB, IHC, ELISA, ICC, FACS and IF. Suitable for Human, Mouse and Rat.

Quick Overview for MTA2 antibody (AA 1-668) (ABIN7599519)

Target: MTA2 (Metastasis Associated 1 Family, Member 2 (MTA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MTA2 Antibody

Binding Specificity: AA 1-668

Purpose: Anti-MTA2/PID Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MTA2/PID Picoband® Antibody (ABIN7599519). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MTA2/PID recombinant protein (Position: M1-D668).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cui, Q., Matsusue, K., Toh, Y., Kono, A., Takiguchi, S. Assignment of the metastasis-associated gene (Mta1) to mouse chromosome band 12F and the metastasis-associated gene 2 (Mta2) to mouse chromosome band 19B by fluorescence in situ hybridization. Cytogenet. Cell Genet. 94: 246-247, 2001. 2. Futamura, M., Nishimori, H., Shiratsuchi, T., Saji, S., Nakamura, Y., Tokino, T. Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1. J. Hum. Genet. 44: 52-56, 1999. 3. Luo, J., Su, F., Chen, D., Shiloh, A., Gu, W. Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature 408: 377-381, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MTA2

Target: MTA2 (Metastasis Associated 1 Family, Member 2 (MTA2))

Alternative Name: MTA2

Background:

Synonyms: Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, MTA1-L1 protein, p53 target protein in deacetylase complex, MTA2, MTA1L1, PID

Tissue Specificity: Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.

Background: Metastasis-associated protein MTA2 is a protein that in humans is encoded by the MTA2 gene. It is mapped to 11q12.3. This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either ly or inly in transcriptional regulation. Their in effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.

Molecular Weight: 75 kDa

Gene ID: 9219

UniProt: O94776

Pathways: Chromatin Binding