USP5 antibody (AA 1-800)

Catalog No. ABIN7599551

The Rabbit Polyclonal anti-USP5 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect USP5 in samples from Human, Mouse and Rat.

Quick Overview for USP5 antibody (AA 1-800) (ABIN7599551)

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-USP5 Antibody

Binding Specificity: AA 1-800

Purpose: Anti-USP5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-USP5 Antibody Picoband® (ABIN7599551). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human USP5 recombinant protein (Position: M1-K800).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ansari-Lari, M. A., Muzny, D. M., Lu, J., Lu, F., Lilley, C. E., Spanos, S., Malley, T., Gibbs, R. A. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13. Genome Res. 6: 314-326, 1996. 2. Reyes-Turcu, F. E., Horton, J. R., Mullally, J. E., Heroux, A., Cheng, X., Wilkinson, K. D. The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. Cell 124: 1197-1208, 2006. 3. Wilkinson, K. D., Tashayev, V. L., O'Connor, L. B., Larsen, C. N., Kasperek, E., Pickart, C. M. Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase T. Biochemistry 34: 14535-14546, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for USP5

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: USP5

Background:

Synonyms: Lymphotactin, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, Small-inducible cytokine C1, Xcl1, Lptn, Ltn, Scyc1

Tissue Specificity: Expressed in activated CD8 (+) T cells. In the thymus, expressed by medullary thymic epithelial cells.

Background: Ubiquitin specific peptidase 5 is an enzyme that in humans is encoded by the USP5 gene. Ubiquitin dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain.

Molecular Weight: 96 kDa

Gene ID: 8078

UniProt: P45974