NDUFA4 antibody (AA 1-81)

Catalog No. ABIN7599555

Product Details anti-NDUFA4 Antibody

Target: NDUFA4 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 4 (NDUFA4))

Binding Specificity: AA 1-81

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-NDUFA4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NDUFA4 Antibody Picoband® (ABIN7599555). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NDUFA4 recombinant protein (Position: M1-F81).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Zhang, Y. , Ge, M. , Chen, Y. , Yang, Y. , Chen, W. , & Wu, D. , et al. (2022). Ndufa4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma. Journal of Bioenergetics and Biomembranes, 54(5), 283-291. 2. Xu, W. , Lai, Y. , Pan, Y. , Tan, M. , Ma, Y. , & Sheng, H. , et al. M6a rna methylation-mediated ndufa4 promotes cell proliferation and metabolism in gastric cancer. Cell Death & Disease. 3. Ramzan, R. , Rhiel, A. , Weber, P. , Kadenbach, B. , & Vogt, S. . (2019). Reversible dimerization of cytochrome c oxidase regulates mitochondrial respiration. Mitochondrion, 49.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NDUFA4

Target: NDUFA4 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 4 (NDUFA4))

Alternative Name: NDUFA4 (NDUFA4 Products)

Background:

Synonyms: Interleukin 1 family member 10 ,Interleukin 1 family, member 10 (Predicted) ,Il1f10 ,Il1f10_predicted ,rCG_45898 ,

Tissue Specificity: Expressed in fetal skin, spleen and tonsil. Expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.

Background: NDUFA4, mitochondrial complex associated is a protein that in humans is encoded by the NDUFA4 gene. The protein encoded by this gene belongs to the complex I 9 kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Molecular Weight: 12 kDa

Gene ID: 4697

UniProt: O00483