MAP1B antibody (AA 102-1849)

Catalog No. ABIN7599633

This anti-MAP1B antibody is a Rabbit Polyclonal antibody detecting MAP1B in IHC, WB, ELISA, FACS and IF. Suitable for Human, Mouse and Rat.

Quick Overview for MAP1B antibody (AA 102-1849) (ABIN7599633)

Target: MAP1B (Microtubule-Associated Protein 1B (MAP1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MAP1B Antibody

Binding Specificity: AA 102-1849

Purpose: Anti-MAP1B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MAP1B Antibody Picoband® (ABIN7599633). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAP1B recombinant protein (Position: H102-R1849). Human MAP1B shares 89.9% and 89.2% amino acid (aa) sequence identity with mouse and rat MAP1B, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Allen, E., Ding, J., Wang, W., Pramanik, S., Chou, J., Yau, V., Yang, Y. Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature 438: 224-228, 2005. 2. Cui, L., Zheng, J., Zhao, Q., Chen, J.-R., Liu, H., Peng, G., Wu, Y., Chen, C., He, Q., Shi, H., Yin, S., Friedman, R. A., Chen, Y., Guan, M.-X. Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss. JCI Insight 5: e136046, 2020. 3. Edelmann, W., Zervas, M., Costello, P., Roback. L., Fischer, I., Hammarback, J. A., Cowan, N., Davies, P., Wainer, B., Kucherlapati, R. Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc. Nat. Acad. Sci. 93: 1270-1275, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MAP1B

Target: MAP1B (Microtubule-Associated Protein 1B (MAP1B))

Alternative Name: MAP1B

Background:

Synonyms: MAP1B, Microtubule-associated protein 1B, MAP-1B [Cleaved into: MAP1B heavy chain, MAP1 light chain LC1]

Background: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.

Molecular Weight: 271 kDa

Gene ID: 4131

UniProt: P46821

Pathways: Microtubule Dynamics, Regulation of Cell Size