BAZ1A antibody (AA 1033-1536)

Catalog No. ABIN7599651

This anti-BAZ1A antibody is a Rabbit Polyclonal antibody detecting BAZ1A in WB, ELISA, IF, FACS and ICC. Suitable for Human, Rat, Mouse and Monkey.

Quick Overview for BAZ1A antibody (AA 1033-1536) (ABIN7599651)

Target: BAZ1A (Bromodomain Adjacent To Zinc Finger Domain, 1A (BAZ1A))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAZ1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-BAZ1A Antibody

Binding Specificity: AA 1033-1536

Purpose: Anti-ACF1/BAZ1A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACF1/BAZ1A Antibody Picoband® (ABIN7599651). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACF1/BAZ1A recombinant protein (Position: E1033-H1536).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Blosser, T. R., Yang, J. G., Stone, M. D., Narlikar, G. J., Zhuang, X. Dynamics of nucleosome remodelling by individual ACF complexes. Nature 462: 1022-1027, 2009. 2. Bochar, D. A., Savard, J., Wang, W., Lafleur, D. W., Moore, P., Cote, J., Shiekhattar, R. A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc. Nat. Acad. Sci. 97: 1038-1043, 2000. 3. Collins, N., Poot, R. A., Kukimoto, I., Garcia-Jimenez, C., Dellaire, G., Varga-Weisz, P. D. An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin. Nature Genet. 32: 627-632, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BAZ1A

Target: BAZ1A (Bromodomain Adjacent To Zinc Finger Domain, 1A (BAZ1A))

Alternative Name: BAZ1A

Background:

Synonyms: Muscarinic acetylcholine receptor M1, CHRM1

Tissue Specificity: Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Background: Bromodomain adjacent to zinc finger domain protein 1A is a protein that in humans is encoded by the BAZ1A gene. The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes.

Molecular Weight: 203 kDa

Gene ID: 11177