Dynactin 1 antibody (AA 1042-1278)

Catalog No. ABIN7599660

This anti-Dynactin 1 antibody is a Rabbit Polyclonal antibody detecting Dynactin 1 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Dynactin 1 antibody (AA 1042-1278) (ABIN7599660)

Target: Dynactin 1 (DCTN1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Dynactin 1 Antibody

Binding Specificity: AA 1042-1278

Purpose: Anti-DCTN1/p150-glued Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DCTN1/p150-glued Antibody Picoband® (ABIN7599660). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DCTN1/p150-glued recombinant protein (Position: K1042-S1278).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Caroppo, P., Le Ber, I., Clot, F., Rivaud-Pechoux, S., Camuzat, A., De Septenville, A., Boutoleau-Bretonniere, C., Mourlon, V., Sauvee, M., Lebouvier, T., Bonnet, A.-M., Levy, R., Vercelletto, M., Brice, A. DCTN1 mutation analysis in families with progressive supranuclear palsy-like phenotypes. JAMA Neurol. 71: 208-215, 2014. 2. Collin, G. B., Nishina, P. M., Marshall, J. D., Naggert, J. K. Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome. Genomics 53: 359-364, 1998. 3. Eaton, B. A., Fetter, R. D., Davis, G. W. Dynactin is necessary for synapse stabilization. Neuron 34: 729-741, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: DCTN1

Background:

Synonyms: Probetacellulin, Betacellulin, BTC, BTC

Tissue Specificity: Highly expressed in testis.

Background: Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene. This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains ly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

Molecular Weight: 150 kDa

Gene ID: 1639

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling