VPS53 antibody (AA 11-699)

Catalog No. ABIN7599731

The Rabbit Polyclonal anti-VPS53 antibody has been validated for WB, ELISA, IF, IHC, ICC and FACS. It is suitable to detect VPS53 in samples from Human, Mouse and Rat.

Quick Overview for VPS53 antibody (AA 11-699) (ABIN7599731)

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-VPS53 Antibody

Binding Specificity: AA 11-699

Purpose: Anti-VPS53 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-VPS53 Antibody Picoband® (ABIN7599731). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human; Mouse; Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human VPS53 recombinant protein (Position: E11-T699).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ben-Zeev, B., Hoffman, C., Lev, D., Watemberg, N., Malinger, G., Brand, N., Lerman-Sagie, T. Progressive cerebellocerebral atrophy: a new syndrome with microcephaly, mental retardation, and spastic quadriplegia. J. Med. Genet. 40: e96, 2003. Note: Electronic Article. 2. Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., Elledge, S. J. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319: 921-926, 2008. 3. Feinstein, M., Flusser, H., Lerman-Sagie, T., Ben-Zeev, B., Lev, D., Agamy, O., Cohen, I., Kadir, R., Sivan, S., Leshinsky-Silver, E., Markus, B., Birk, O. S. VPS53 mutations cause progressive cerebello-cerebral atrophy type 2 (PCCA2). J. Med. Genet. 51: 303-308, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for VPS53

Target: VPS53 (Vacuolar Protein Sorting 53 Homolog (VPS53))

Alternative Name: VPS53

Background:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: Vacuolar protein sorting 53 homolog (S. cerevisiae) is a protein that in humans is encoded by the VPS53 gene. This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.

Molecular Weight: 100 kDa

Gene ID: 55275