Rab4 antibody (AA 110-216)

Catalog No. ABIN7599733

This Rabbit Polyclonal antibody specifically detects Rab4 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Rab4 antibody (AA 110-216) (ABIN7599733)

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rab4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Rab4 Antibody

Binding Specificity: AA 110-216

Purpose: Anti-Rab4/RAB4A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Rab4/RAB4A Antibody Picoband® (ABIN7599733). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Rab4/RAB4A recombinant protein (Position: D110-C216).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Barbosa, M. D. F. S., Johnson, S. A., Achey, K., Gutierrez, M., Wakeland, E. K., Zerial, M., Kingsmore, S. F. The Rab protein family: genetic mapping of six Rab genes in the mouse. Genomics 30: 439-444, 1995. 2. Bielli, A., Thornqvist, P.-O., Hendrick, A. G., Finn, R., Fitzgerald, K., McCaffrey, M. W. The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1. Biochem. Biophys. Res. Commun. 281: 1141-1153, 2001. 3. Goueli, B. S., Powell, M. B., Finger, E. C., Pfeffer, S. R. TBC1D16 is a Rab4A GTPase activating protein that regulates receptor recycling and EGF receptor signaling. Proc. Nat. Acad. Sci. 109: 15787-15792, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Rab4

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Alternative Name: RAB4A

Background:

Synonyms: Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, hKCC2, Neuronal K-Cl cotransporter, SLC12A5, KCC2, KIAA1176

Tissue Specificity: Brain specific. Detected in neuronal cells.

Background: This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

Molecular Weight: 24 kDa

Gene ID: 5867

UniProt: P20338