HFE antibody (AA 112-227)

Catalog No. ABIN7599746

The Rabbit Polyclonal anti-HFE antibody has been validated for WB, ELISA, FACS and IHC. It is suitable to detect HFE in samples from Mouse and Rat.

Quick Overview for HFE antibody (AA 112-227) (ABIN7599746)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-HFE Antibody

Binding Specificity: AA 112-227

Purpose: Anti-Hfe Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Hfe Antibody Picoband® (ABIN7599746). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Hfe recombinant protein (Position: N112-H227).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Aguilar-Martinez, P., Bismuth, M., Picot, M. C., Thelcide, C., Pageaux, G.-P., Blanc, F., Blanc, P., Schved, J.-F., Larrey, D. Variable phenotypic presentation of iron overload in H63D homozygotes: are genetic modifiers the cause? Gut 48: 836-842, 2001. 2. Allen, K. J., Gurrin, L. C., Constantine, C. C., Osborne, N. J., Delatycki, M. B., Nicoll, A. J., McLaren, C. E., Bahlo, M., Nisselle, A. E., Vulpe, C. D., Anderson, G. J., Southey, M. C., Giles, G. G., English, D. R., Hopper, J. L., Olynyk, J. K., Powell, L. W., Gertig, D. M. Iron-overload-related disease in HFE hereditary hemochromatosis. New Eng. J. Med. 358: 221-230, 2008. 3. Andersen, R. V., Tybjaerg-Hansen, A., Appleyard, M., Birgens, H., Nordestgaard, B. G. Hemochromatosis mutations in the general population: iron overload progression rate. Blood 103: 2914-2919, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: Hfe

Background:

Synonyms: Hereditary hemochromatosis protein,HLA-H,HFE,HLAH,

Tissue Specificity: Expressed in all tissues tested except brain.

Background: Human homeostatic iron regulator protein, also known as the HFE protein (High FE2+), is a protein which in humans is encoded by the HFE gene. Enables transferrin receptor binding activity. Involved in positive regulation of gene expression. Acts upstream of or within hormone biosynthetic process and multicellular organismal iron ion homeostasis. Part of HFE-transferrin receptor complex. Is expressed in brain, choroid invagination, diencephalon roof plate, medulla oblongata part of 4th ventricle choroid plexus, and metencephalon part of 4th ventricle choroid plexus. Used to study hemochromatosis type 1. Human ortholog(s) of this gene implicated in several diseases, including acute porphyria (multiple), arthritis (multiple), bone marrow cancer (multiple), hemochromatosis (multiple), and liver disease (multiple). Orthologous to human HFE (homeostatic iron regulator).

Molecular Weight: 40-58 kDa

Gene ID: 15216

UniProt: P70387

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process