TWF1 antibody (AA 116-350)

Catalog No. ABIN7599771

The Rabbit Polyclonal anti-TWF1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect TWF1 in samples from Human.

Quick Overview for TWF1 antibody (AA 116-350) (ABIN7599771)

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TWF1 Antibody

Binding Specificity: AA 116-350

Purpose: Anti-TWF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TWF1 Antibody Picoband® (ABIN7599771). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TWF1 recombinant protein (Position: H116-D350).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Beeler, J. F., LaRochelle, W. J., Chedid, M., Tronick, S. R., Aaronson, S. A. Prokaryotic expression cloning of a novel human tyrosine kinase. Molec. Cell. Biol. 14: 982-988, 1994. 2. Meacham, C. E., Ho, E. E., Dubrovsky, E., Gertler, F. B., Hemann, M. T. In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression. Nature Genet. 41: 1133-1137, 2009. 3. Paavilainen, V. O., Hellman, M., Helfer, E., Bovellan, M., Annila, A., Carlier, M.-F., Permi, P., Lappalainen, P. Structural basis and evolutionary origin of actin filament capping by twinfilin. Proc. Nat. Acad. Sci. 104: 3113-3118, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TWF1

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: TWF1

Background:

Synonyms: Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7

Tissue Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

Background: Twinfilin-1 is a protein that in humans is encoded by the TWF1 gene. This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Molecular Weight: 42 kDa

Gene ID: 5756

UniProt: Q12792

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location