GRSF1 antibody (AA 119-405)

Catalog No. ABIN7599803

This Rabbit Polyclonal antibody specifically detects GRSF1 in WB, ELISA, IHC, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for GRSF1 antibody (AA 119-405) (ABIN7599803)

Target: GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-GRSF1 Antibody

Binding Specificity: AA 119-405

Purpose: Anti-GRSF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GRSF1 Antibody Picoband® (ABIN7599803). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRSF1 recombinant protein (Position: Q119-M405).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Banga, S. S., Ozer, H. L., Wilusz, J. Assignment of GRSF1 encoding a poly(A)+ mRNA binding protein to human chromosome 4q13. Cytogenet. Cell Genet. 73: 295-296, 1996. 2. Qian, Z., Wilusz, J. GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element. Nucleic Acids Res. 22: 2334-2343, 1994. 3. Qian, Z., Wilusz, J. An RNA-binding protein specifically interacts with a functionally important domain of the downstream element of the simian virus 40 late polyadenylation signal. Molec. Cell. Biol. 11: 5312-5320, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRSF1

Target: GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))

Alternative Name: GRSF1

Background:

Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16

Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Background: G-rich sequence factor 1 (GRSF1) is a regulator of post-transcriptional mitochondrial gene expression. The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 53 kDa

Gene ID: 2926

UniProt: Q12849