TEAD2 antibody (AA 125-398)

Catalog No. ABIN7599879

The Rabbit Polyclonal anti-TEAD2 antibody has been validated for ELISA, WB, IHC and FACS. It is suitable to detect TEAD2 in samples from Human, Mouse and Rat.

Quick Overview for TEAD2 antibody (AA 125-398) (ABIN7599879)

Target: TEAD2 (TEA Domain Family Member 2 (TEAD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEAD2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-TEAD2 Antibody

Binding Specificity: AA 125-398

Purpose: Anti-ETF/TEAD2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ETF/TEAD2 Antibody Picoband® (ABIN7599879). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ETF/TEAD2 recombinant protein (Position: K125-M398).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jacquemin, P., Depetris, D., Mattei, M.-G., Martial, J. A., Davidson, I. Localization of human transcription factor TEF-4 and TEF-5 (TEAD2, TEAD3) genes to chromosomes 19q13.3 and 6p21.2 using fluorescence in situ hybridization and radiation hybrid analysis. Genomics 55: 127-129, 1999. 2. Jacquemin, P., Hwang, J.-J., Martial, J. A., Dolle, P., Davidson, I. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain. J. Biol. Chem. 271: 21775-21785, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TEAD2

Target: TEAD2 (TEA Domain Family Member 2 (TEAD2))

Alternative Name: TEAD2

Background:

Synonyms: Homer protein homolog 3,Homer-3,HOMER3,

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: TEAD2 (ETF, ETEF-1, TEF-4), together with TEAD1, defines a novel family of transcription factors, the TEAD family, highly conserved through evolution. TEAD proteins were notably found in Drosophila (Scalloped), C. elegans (egl -44), S. cerevisiae and A. nidulans. TEAD2 has been less studied than TEAD1 but a few studies revealed its role during development. TEAD2 is a member of the mammalian TEAD transcription factor family (initially named the transcriptional enhancer factor (TEF) family), which contain the TEA/ATTS DNA-binding domain.[7] Members of the family in mammals are TEAD1, TEAD2, TEAD3, TEAD4.

Molecular Weight: 49 kDa

Gene ID: 8463

UniProt: Q15562

Pathways: Regulation of Lipid Metabolism by PPARalpha, Tube Formation