TTC39B antibody (AA 129-682)

Catalog No. ABIN7599918

The Rabbit Polyclonal anti-TTC39B antibody has been validated for WB, ELISA and FACS. It is suitable to detect TTC39B in samples from Human, Mouse and Rat.

Quick Overview for TTC39B antibody (AA 129-682) (ABIN7599918)

Target: TTC39B (Tetratricopeptide Repeat Domain 39B (TTC39B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTC39B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TTC39B Antibody

Binding Specificity: AA 129-682

Purpose: Anti-TTC39B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TTC39B Antibody Picoband® (ABIN7599918). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TTC39B recombinant protein (Position: K129-D682).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/1/2010. 2. Hsieh, J., Koseki, M., Molusky, M. M., Yakushiji, E., Ichi, I., Westerterp, M., Iqbal, J., Chan, R. B., Abramowicz, S., Tascau, L., Takiguchi, S., Yamashita, S., Welch, C. L., Di Paolo, G., Hussain, M. M., Lefkowitch, J. H., Rader, D. J., Tall, A. R. TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis. Nature 535: 303-307, 2016. 3. Teslovich, T. M., Musunuru, K., Smith, A. V., Edmondson, A. C., Stylianou, I. M., Koseki, M., Pirruccello, J. P., Ripatti, S., Chasman, D. I., Willer, C. J., Johansen, C. T., Fouchier, S. W., and 197 others. Biological, clinical and population relevance of 95 loci for blood lipids. Nature 466: 707-713, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TTC39B

Target: TTC39B (Tetratricopeptide Repeat Domain 39B (TTC39B))

Alternative Name: TTC39B

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: Tetratricopeptide repeat protein 39B is a protein that in humans is encoded by the TTC39B gene. TTC39B protein contains two TPR repeats (aa 39 3-426 and 626 -659). TCC39B protein is the product of a high density lipoprotein (HDL) gene that promotes the ubiquitination and degradation of liver X receptor (LXR). TTC39B null or deficient mice challenged with high fat/cholesterol/bile salt diet exhibit increased LX R protein and target gene expression. TTC39B deficiency is reported to stabilize LXR by reducing its polyubiquitination and proteasomal degradation. Hence it reduces the incidence of atherosclerosis and steatohepatitis. TTC39B deficiency mice display higher levels of HDL and Apoplipoprotein A-1 and lower levels of hepatic triglyceride synthesis.However it does not affect glucose tolerance or hepatic gluconeogenesis.

Molecular Weight: 70 kDa

Gene ID: 158219