MICALL2 antibody (AA 132-741)

Catalog No. ABIN7599972

The Rabbit Polyclonal anti-MICALL2 antibody has been validated for WB, ELISA, FACS and IHC. It is suitable to detect MICALL2 in samples from Human.

Quick Overview for MICALL2 antibody (AA 132-741) (ABIN7599972)

Target: MICALL2 (MICAL-Like 2 (MICALL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICALL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-MICALL2 Antibody

Binding Specificity: AA 132-741

Purpose: Anti-MICALL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MICALL2 Antibody Picoband® (ABIN7599972). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MICALL2 recombinant protein (Position: K132-Q741).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sakane, A., Yano, T. A., Uchihashi, T., Horikawa, K., Hara, Y., Imoto, I., ... & Sasaki, T. (2021). JRAB/MICAL-L2 undergoes liquid-liquid phase separation to form tubular recycling endosomes. Communications biology, 4(1), 551. 2. Zhu, L. Y., Zhang, W. M., Yang, X. M., Cui, L., Li, J., Zhang, Y. L., ... & Zhang, Z. G. (2015). Silencing of MICAL-L2 suppresses malignancy of ovarian cancer by inducing mesenchymal-epithelial transition. Cancer letters, 363(1), 71-82. 3. Min, P., Zhao, S., Liu, L., Zhang, Y., Ma, Y., Zhao, X., ... & Du, J. (2019). MICAL-L2 potentiates Cdc42-dependent EGFR stability and promotes gastric cancer cell migration. Journal of cellular and molecular medicine, 23(6), 4475-4488.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MICALL2

Target: MICALL2 (MICAL-Like 2 (MICALL2))

Alternative Name: MICALL2

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: Enables filamin binding activity. Involved in positive regulation of protein targeting to mitochondrion. Predicted to be located in several cellular components, including bicellular tight junction, neuron projection, and recycling endosome. Predicted to colocalize with stress fiber.

Molecular Weight: 109 kDa

Gene ID: 79778