Neurogenin 3 antibody (AA 137-214)

Catalog No. ABIN7599987

The Rabbit Polyclonal anti-Neurogenin 3 antibody has been validated for WB, ELISA and FACS. It is suitable to detect Neurogenin 3 in samples from Human and Mouse.

Quick Overview for Neurogenin 3 antibody (AA 137-214) (ABIN7599987)

Target: Neurogenin 3 (NEUROG3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurogenin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Neurogenin 3 Antibody

Binding Specificity: AA 137-214

Purpose: Anti-NEUROG3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NEUROG3 Antibody Picoband® (ABIN7599987). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NEUROG3 recombinant protein (Position: Q137-L214). Human NEUROG3 shares 72.2% amino acid (aa) sequence identity with mouse NEUROG3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Gradwohl, G., Dierich, A., LeMeur, M., Guillemot, F. Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas. Proc. Nat. Acad. Sci. 97: 1607-1611, 2000. 2. Kim, S.-H., Warram, J. H., Krolewski, A. S., Doria, A. Mutation screening of the neurogenin-3 gene in autosomal dominant diabetes. J. Clin. Endocr. Metab. 86: 2320-2322, 2001. 3. Lee, C. S., Perreault, N., Brestelli, J. E., Kaestner, K. H. Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity. Genes Dev. 16: 1488-1497, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Alternative Name: NEUROG3

Background:

Synonyms: NEUROG3, ATOH5, BHLHA7, NGN3, Neurogenin-3, NGN-3, Class A basic helix-loop-helix protein 7, bHLHa7, Protein atonal homolog 5

Background: The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).

Molecular Weight: 23 kDa

Gene ID: 50674

UniProt: Q9Y4Z2