CD147 antibody (AA 138-323)

Catalog No. ABIN7599999

The Mouse Monoclonal anti-CD147 antibody has been validated for WB, FACS, IHC and IF. It is suitable to detect CD147 in samples from Human and Mouse.

Quick Overview for CD147 antibody (AA 138-323) (ABIN7599999)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 7H5E7

Product Details anti-CD147 Antibody

Binding Specificity: AA 138-323

Purpose: Anti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-/Emmprin Antibody Picoband® (monoclonal, 7H5E7) (ABIN7599999). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Guo, H., Majmudar, G., Jensen, T. C., Biswas, C., Toole, B. P., Gordon, M. K.Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases.Gene 220: 99-108, 1998. 2. Zhou, S., Zhou, H., Walian, P. J., Jap, B. K.CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: BSG

Background:

Synonyms: Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4

Tissue Specificity: Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

Background: Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Molecular Weight: 35-60 kDa

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins