FOXA2 antibody (AA 139-446)

Catalog No. ABIN7600003

This anti-FOXA2 antibody is a Rabbit Polyclonal antibody detecting FOXA2 in WB, ELISA, IHC, IF, FACS and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for FOXA2 antibody (AA 139-446) (ABIN7600003)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FOXA2 Antibody

Binding Specificity: AA 139-446

Purpose: Anti-FOXA2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FOXA2 Antibody Picoband® (ABIN7600003). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FOXA2 recombinant protein (Position: Q139-Q446).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Avraham, K. B., Prezioso, V. R., Chen, W. S., Lai, E., Sladek, F. M., Zhong, W., Darnell, J. E., Jr., Jenkins, N. A., Copeland, N. G. Murine chromosomal location of four hepatocyte-enriched transcription factors: HNF-3-alpha, HNF-3-beta, HNF-3-gamma, and HNF-4. Genomics 13: 264-268, 1992. 2. Bochkis, I. M., Rubins, N. E., White, P., Furth, E. E., Friedman, J. R., Kaestner, K. H. Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress. Nature Med. 14: 828-836, 2008. 3. Deleuze, J. F., Dhorne, S., Hazan, J., Borghi, E., Raynaud, N., Pollet, N., Meunier-Rotival, M., Deschatrette, J., Alagille, D., Hadchouel, M. Deleted chromosome 20 from a patient with Alagille syndrome isolated in a cell hybrid through leucine transport selection: study of three candidate genes. Mammalian Genome 5: 663-669, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2

Background:

Synonyms: Tyrosine-protein kinase Tec, TEC, PSCTK4

Tissue Specificity: Expressed in a wide range of cells, including hematopoietic cell lines like myeloid, B-, and T-cell lineages.

Background: Forkhead box protein A2 (FOXA2), also known as hepatocyte nuclear factor 3-beta (HNF-3B), is a transcription factor that plays an important role during development, in mature tissues and, when dysregulated or mutated, also in cancer. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.

Molecular Weight: 48 kDa

Gene ID: 3170

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process