AHCYL1 antibody (AA 14-57)

Catalog No. ABIN7600029

Product Details anti-AHCYL1 Antibody

Target: AHCYL1 (Adenosylhomocysteinase-Like 1 (AHCYL1))

Binding Specificity: AA 14-57

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AHCYL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) (ABIN7600029). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IRBIT/AHCYL1 recombinant protein (Position: E14-K57).

Clone: 2E7D9

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ando, H., Mizutani, A., Kiefer, H., Tsuzurugi, D., Michikawa, T., Mikoshiba, K. IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor. Molec. Cell 22: 795-806, 2006. 2. Ando, H., Mizutani, A., Matsu-ura, T., Mikoshiba, K. IRBIT, a novel inositol 1,4,5-trisphosphate (IP3) receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor. J. Biol. Chem. 278: 10602-10612, 2003. 3. Ando, H., Mizutani, A., Mikoshiba, K. An IRBIT homologue lacks binding activity to inositol 1,4,5-trisphosphate receptor due to the unique N-terminal appendage. J. Neurochem. 109: 539-550, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AHCYL1

Target: AHCYL1 (Adenosylhomocysteinase-Like 1 (AHCYL1))

Alternative Name: AHCYL1 (AHCYL1 Products)

Background:

Synonyms: T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Putative adenosylhomocysteinase 2 is an enzyme that in humans is encoded by the AHCYL1 gene. The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 59 kDa

Gene ID: 10768

UniProt: O43865