TRIM36 antibody (AA 143-642)

Catalog No. ABIN7600059

The Rabbit Polyclonal anti-TRIM36 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect TRIM36 in samples from Human, Mouse and Rat.

Quick Overview for TRIM36 antibody (AA 143-642) (ABIN7600059)

Target: TRIM36 (Tripartite Motif Containing 36 (TRIM36))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM36 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TRIM36 Antibody

Binding Specificity: AA 143-642

Purpose: Anti-TRIM36 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIM36 Antibody Picoband® (ABIN7600059). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRIM36 recombinant protein (Position: E143-K642).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Balint, I., Muller, A., Nagy, A., Kovacs, G. Cloning and characterisation of the RBCC728/TRIM36 zinc-binding protein from the tumor suppressor gene region at chromosome 5q22.3. Gene 332: 45-50, 2004. 2. Reymond, A., Meroni, G., Fantozzi, A., Merla, G., Cairo, S., Luzi, L., Riganelli, D., Zanaria, E., Messali, S., Cainarca, S., Guffanti, A., Minucci, S., Pelicci, P. G., Ballabio, A. The tripartite motif family identifies cell compartments. EMBO J. 20: 2140-2151, 2001. 3. Singh, N., Kumble Bhat, V., Tiwari, a., Kodaganur, S. G., Tontanahal, S. J., Sarda, A., Malini, K. V., Kumar, A. A homozygous mutation in TRIM36 causes autosomal recessive anencephaly in an Indian family. Hum. Molec. Genet. 26: 1104-1114, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIM36

Target: TRIM36 (Tripartite Motif Containing 36 (TRIM36))

Alternative Name: TRIM36

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Molecular Weight: 83 kDa

Gene ID: 55521