PHEX antibody (AA 145-718)

Catalog No. ABIN7600072

This anti-PHEX antibody is a Rabbit Polyclonal antibody detecting PHEX in ELISA, WB, FACS and IHC. Suitable for Human, Rat, Mouse and Monkey.

Quick Overview for PHEX antibody (AA 145-718) (ABIN7600072)

Target: PHEX (Phosphate Regulating Endopeptidase Homolog, X-Linked (PHEX))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHEX antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-PHEX Antibody

Binding Specificity: AA 145-718

Purpose: Anti-PHEX Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PHEX Antibody Picoband® (ABIN7600072). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHEX recombinant protein (Position: E145-N718).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Christie, P. T., Harding, B., Nesbit, M. A., Whyte, M. P., Thakker, R. V. X-linked hypophosphatemia attributable to pseudoexons of the PHEX gene. J. Clin. Endocr. Metab. 86: 3840-3844, 2001. 2. Dixon, P. H., Christie, P. T., Wooding, C., Trump, D., Grieff, M., Holm, I., Gertner, J. M., Schmidtke, J., Shah, B., Shaw, N., Smith, C., Tau, C., Schlessinger, D., Whyte, M. P., Thakker, R. V. Mutational analysis of PHEX gene in X-linked hypophosphatemia. J. Clin. Endocr. Metab. 83: 3615-3623, 1998. 3. Econs, M. J., Friedman, N. E., Rowe, P. S. N., Speer, M. C., Francis, F., Strom, T. M., Oudet, C., Smith, J. A., Ninomiya, J. T., Lee, B. E., Bergen, H. A PHEX gene mutation is responsible for adult-onset vitamin D-resistant hypophosphatemic osteomalacia: evidence that the disorder is not a distinct entity from X-linked hypophosphatemic rickets. J. Clin. Endocr. Metab. 83: 3459-3462, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PHEX

Target: PHEX (Phosphate Regulating Endopeptidase Homolog, X-Linked (PHEX))

Alternative Name: PHEX

Background:

Synonyms: Phosphate-regulating neutral endopeptidase PHEX, Metalloendopeptidase homolog PEX, Vitamin D-resistant hypophosphatemic rickets protein, X-linked hypophosphatemia protein, HYP, PHEX, PEX

Tissue Specificity: Specifically expressed in ovary. Expressed at low levels in kidney.

Background: Phosphate-regulating neutral endopeptidase, X-linked also known as phosphate-regulating gene with homologies to endopeptidases on the X chromosome or metalloendopeptidase homolog PEX is an enzyme that in humans is encoded by the PHEX gene. It is mapped to Xp22.11. The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants.

Molecular Weight: 86 kDa

Gene ID: 5251

UniProt: P78562