CD28 antibody (AA 15-214)

Catalog No. ABIN7600101

The Rabbit Polyclonal anti-CD28 antibody has been validated for FACS, WB and ELISA. It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (AA 15-214) (ABIN7600101)

Target: CD28

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-CD28 Antibody

Binding Specificity: AA 15-214

Purpose: Anti-CD28 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD28 Antibody Picoband® (ABIN7600101). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD28 recombinant protein (Position: Q15-D214).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Akieda, Y., Wakamatsu, E., Nakamura, T., Ishida, Y., Ogawa, S., Abe, R. Defects in regulatory T cells due to CD28 deficiency induce a qualitative change of allogeneic immune response in chronic graft-versus-host disease. J. Immun. 194: 4162-4174, 2015. 2. Andres, P. G., Howland, K. C., Nirula, A., Kane, L. P., Barron, L., Dresnek, D., Sadra, A., Imboden, J., Weiss, A., Abbas, A. K. Distinct regions in the CD28 cytoplasmic domain are required for T helper type 2 differentiation. Nature Immun. 5: 435-442, 2004. 3. Aruffo, A., Seed, B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc. Nat. Acad. Sci. 84: 8573-8577, 1987.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background:

Synonyms: Haptoglobin, Zonulin, HP

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 60 kDa

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response