XIAP antibody (AA 15-244)

Catalog No. ABIN7600103

This Mouse Monoclonal antibody specifically detects XIAP in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for XIAP antibody (AA 15-244) (ABIN7600103)

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3G2G1

Product Details anti-XIAP Antibody

Binding Specificity: AA 15-244

Purpose: Anti-XIAP Antibody Picoband® (monoclonal, 3G2G1)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-XIAP Antibody Picoband® (monoclonal, 3G2G1) (ABIN7600103). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human XIAP recombinant protein (Position: A15-V244). Human XIAP shares 89.4% and 90.7% amino acid (aa) sequence identity with mouse and rat XIAP, respectively.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
1. Cummins, J. M., Kohli, M., Rago, C., Kinzler, K. W., Vogelstein, B., Bunz, F.X-linked inhibitor of apoptosis protein (XIAP) is a nonredundant modulator of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human cancer cells.Cancer Res. 64: 3006-3008, 2004. 2. Deveraux, Q. L., Takahashi, R., Salvesen, G. S., Reed, J. C.X-linked IAP is a inhibitor of cell-death proteases.Nature 388: 300-304, 1997. 3. Kim, J., Park, J., Choi, S., Chi, S.-G., Mowbray, A. L., Jo, H., Park, H.X-linked inhibitor of apoptosis protein is an important regulator of vascular endothelial growth factor-dependent bovine aortic endothelial cell survival.Circ. Res. 102: 896-904, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP

Background:

Synonyms: T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1

Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

Background: XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death. It is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants.

Molecular Weight: 54 kDa

Gene ID: 331

UniProt: P98170

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis