TBR1 antibody (AA 15-682)

Catalog No. ABIN7600119

The Rabbit Polyclonal anti-TBR1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect TBR1 in samples from Human, Mouse and Rat.

Quick Overview for TBR1 antibody (AA 15-682) (ABIN7600119)

Target: TBR1 (T-Box Brain Gene 1 (TBR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-TBR1 Antibody

Binding Specificity: AA 15-682

Purpose: Anti-TBR1 Antibody Picoband®

Characteristics: Anti-TBR1 Antibody Picoband® (ABIN7600119). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TBR1 recombinant protein (Position: K15-S682). Human TBR1 shares 99.3% amino acid (aa) sequence identity with mouse TBR1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bulfone, A., Smiga, S. M., Shimamura, K., Peterson, A., Puelles, L., Rubenstein, J. L. R. T-brain-1: a homolog of Brachyury whose expression defines molecularly distinct domains within the cerebral cortex. Neuron 15: 63-78, 1995. 2. den Hoed, J., Sollis, E., Venselaar, H., Estruch, S. B., Deriziotis, P., Fisher, S. E. Functional characterization of TBR1 variants in neurodevelopmental disorder. Sci. Rep. 8: 14279, 2018. 3. Deriziotis, P., O'Roak, B. J., Graham, S. A., Estruch, S. B., Dimitropoulou, D., Bernier, R. A., Gerdts, J., Shendure, J., Eichler, E. E., Fisher, S. E. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature Commun. 5: 4954, 2014. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TBR1

Target: TBR1 (T-Box Brain Gene 1 (TBR1))

Alternative Name: TBR1

Background: T-box, brain, 1 is a transcription factor protein important in vertebrate embryo development. It is encoded by the TBR1 gene. This gene is a member of a conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of numerous developmental processes. In mouse, the ortholog of this gene is expressed in the cerebral cortex, hippocampus, amygdala and olfactory bulb and is thought to play an important role in neuronal migration and axonal projection. In mouse, the C-terminal region of this protein was found to be necessary and sufficient for association with the guanylate kinase domain of calcium/calmodulin-dependent serine protein kinase.

Molecular Weight: 74 kDa

Gene ID: 10716

UniProt: Q16650