UBE2O antibody (AA 150-1283)

Catalog No. ABIN7600121

This Rabbit Polyclonal antibody specifically detects UBE2O in WB, ELISA and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for UBE2O antibody (AA 150-1283) (ABIN7600121)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2O antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-UBE2O Antibody

Binding Specificity: AA 150-1283

Purpose: Anti-UBE2O Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UBE2O Antibody Picoband® (ABIN7600121). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBE2O recombinant protein (Position: R150-A1283).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mashtalir, N., Daou, S., Barbour, H., Sen, N. N., Gagnon, J., Hammond-Martel, I., Dar, H. H., Therrien, M., Affar, E. B. Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Molec. Cell 54: 392-406, 2014. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000. 3. Nguyen, A. T., Prado, M. A., Schmidt, P. J., Sendamarai, A. K., Wilson-Grady, J. T., Min, M., Campagna, D. R., Tian, G., Shi, Y., Dederer, V., Kawan, M., Kuehnle, N., Paulo, J. A., Yao, Y., Weiss, M. J., Justice, M. J., Gygi, S. P., Fleming, M. D., Finley, D. UBE2O remodels the proteome during terminal erythroid differentiation. Science 357: eaan0218, 2017. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O

Background:

Synonyms: Homer protein homolog 3,Homer-3,HOMER3,

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway, protein ubiquitination, and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.

Molecular Weight: 200 kDa

Gene ID: 63893

UniProt: Q9C0C9