SCLY antibody (AA 157-445)

Catalog No. ABIN7600155

The Rabbit Polyclonal anti-SCLY antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect SCLY in samples from Human, Mouse and Rat.

Quick Overview for SCLY antibody (AA 157-445) (ABIN7600155)

Target: SCLY (Selenocysteine Lyase (SCLY))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCLY antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SCLY Antibody

Binding Specificity: AA 157-445

Purpose: Anti-SCLY Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SCLY Antibody Picoband® (ABIN7600155). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SCLY recombinant protein (Position: E157-A445).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mihara, H., Kurihara, T., Watanabe, T., Yoshimura, T., Esaki, N. cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase: candidate for selenium delivery protein in selenoprotein synthesis. J. Biol. Chem. 275: 6195-6200, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SCLY

Target: SCLY (Selenocysteine Lyase (SCLY))

Alternative Name: SCLY

Background:

Synonyms: Tudor domain-containing protein 3, TDRD3

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: SCLY(Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al.(2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Molecular Weight: 48 kDa

Gene ID: 51540

UniProt: Q96I15

Pathways: Stem Cell Maintenance