TRIT1 antibody (AA 16-415)

Catalog No. ABIN7600178

This Rabbit Polyclonal antibody specifically detects TRIT1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for TRIT1 antibody (AA 16-415) (ABIN7600178)

Target: TRIT1 (tRNA Isopentenyltransferase 1 (TRIT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TRIT1 Antibody

Binding Specificity: AA 16-415

Purpose: Anti-IPT/TRIT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IPT/TRIT1 Antibody Picoband® (ABIN7600178). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IPT/TRIT1 recombinant protein (Position: L16-K415).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Golovko, A., Hjalm, G., Sitbon, F., Nicander, B. Cloning of a human tRNA isopentenyl transferase. Gene 258: 85-93, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/19/2018. 3. Kernohan, K. D., Dyment, D. A., Pupavac, M., Cramer, Z., McBride, A., Bernard, G., Straub, I., Tetreault, M., Hartley, T., Huang, L., Sell, E., Majewski, J., and 14 others. Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene. Hum. Mutat. 38: 511-516, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIT1

Target: TRIT1 (tRNA Isopentenyltransferase 1 (TRIT1))

Alternative Name: TRIT1

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8,

Tissue Specificity: Ubiquitous.

Background: tRNA isopentenyltransferase, mitochondrial is an enzyme that in humans is encoded by the TRIT1 gene. This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional.

Molecular Weight: 53 kDa

Gene ID: 54802

UniProt: Q9H3H1