PRKCSH antibody (AA 16-506)

Catalog No. ABIN7600183

This anti-PRKCSH antibody is a Rabbit Polyclonal antibody detecting PRKCSH in WB, ELISA, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PRKCSH antibody (AA 16-506) (ABIN7600183)

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKCSH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PRKCSH Antibody

Binding Specificity: AA 16-506

Purpose: Anti-PRKCSH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRKCSH Antibody Picoband® (ABIN7600183). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRKCSH recombinant protein (Position: E16-L506).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cornec-Le Gall, E., Torres, V. E., Harris, P. C. Genetic complexity of autosomal dominant polycystic kidney and liver diseases. J. Am. Soc. Nephrol. 29: 13-23, 2018. 2. Drenth, J. P. H., te Morsche, R. H. M., Smink, R., Bonifacino, J. S., Jansen, J. B. M. J. Germline mutations in PRKCSH are associated with autosomal dominant polycystic liver disease. Nature Genet. 33: 345-348, 2003. 3. Fedeles, S. V., Tian, X., Gallagher, A.-R., Mitobe, M., Nishio, S., Lee, S. H., Cai, Y., Geng, L., Crews, C. M., Somlo, S. A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation. Nature Genet. 43: 639-647, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Alternative Name: PRKCSH

Background:

Synonyms: Fms-related tyrosine kinase 3 ligand ,Flt3lg ,

Background: Glucosidase 2 subunit beta is an enzyme that in humans is encoded by the PRKCSH gene. This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

Molecular Weight: 90 kDa

Gene ID: 5589

UniProt: P14314

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process